If you require oligos that are approved for use in molecular diagnostic applications, or if you are interested in our OEM services, click here. Chat now Submit question. Toggle navigation. Order Menu.
Antisense oligonucleotide therapy - Latest research and news | Nature
More functional genomics products RNA interference. Antisense oligos. Antisense oligonucleotides. Order now. Technical overview Antisense oligonucleotides ASOs are used to inhibit gene expression levels both in vitro and in vivo. Figure 1.
Antisense oligo—mediated cleavage of the target by RNase H. Phosphorothioates and chimeric oligos. Antisense therapy is a form of treatment for genetic disorders or infections. When the genetic sequence of a particular gene is known to cause a particular disease, it is possible to synthesize a strand of nucleic acid DNA , RNA or a chemical analogue that will bind to the messenger RNA mRNA produced by that gene and inactivate it, effectively turning that gene "off".
This is because mRNA has to be single stranded for it to be translated. Alternatively, the strand might be targeted to bind a splicing site on pre-mRNA and modify the exon content of an mRNA. Antisense oligonucleotides have been researched as potential drugs    for diseases such as cancers including lung cancer , colorectal carcinoma , pancreatic carcinoma , malignant glioma and malignant melanoma , diabetes , amyotrophic lateral sclerosis ALS , Parkinson's disease ,  Duchenne muscular dystrophy , spinal muscular atrophy , Ataxia-telangiectasia in vitro and diseases such as asthma , arthritis and pouchitis with an inflammatory component.
As of , several antisense drugs have been approved by the U. Food and Drug Administration FDA : fomivirsen as a treatment for cytomegalovirus retinitis , mipomersen for homozygous familial hypercholesterolemia , eteplirsen for Duchenne muscular dystrophy, and nusinersen for spinal muscular atrophy. Also in , German physicians reported on a dose-escalation study for the compound AP a phosphorothioate antisense oligodeoxynucleotide specific for the mRNA of human transforming growth factor TGF-beta2 in patients with high grade gliomas. At the time of the report, the median overall survival had not been obtained and the authors hinted at a potential cure.
Fomivirsen marketed as Vitravene , was approved by the U.
- The Mayor of Casterbridge (Bantam Classics)!
- Molecular Mechanisms of Antisense Oligonucleotides | Nucleic Acid Therapeutics.
- Geometric Design Practices for Resurfacing, Restoration, and Rehabilitation - A Synthesis of Highway Practice;
- Antisense Research and Application.
- CURRENT Diagnosis & Treatment Nephrology & Hypertension.
- chapter and author info?
- Login using.
FDA in Aug as a treatment for cytomegalovirus retinitis. In January mipomersen marketed as Kynamro was approved by the FDA for the treatment of homozygous familial hypercholesterolemia. These drugs, AVI  and AVI are novel analogs based on AVI's PMO antisense chemistry in which anti-viral potency is enhanced by the addition of positively charged components to the morpholino oligomer chain. Preclinical results of AVI and AVI demonstrated reproducible and high rates of survival in non-human primates challenged with a lethal infection of the Ebola and Marburg viruses, respectively.
Starting in , researchers in the US have been conducting research on using antisense technology to combat HIV. Yoo, H. Pae, and C. Holt, C. Rovinson-Benion, and R. Kairemo, A. Jekunen, and M. Tenhunen , Dosimetry and Optimization of in vivo Targeting with Radiolabeled Antisense Oligodeoxynucleotides oligonucleotide radiotherapy.
Offensperger, W. Offensperger, C. Thoma, D. Moradpour, F. Raizada, H.
Antisense Technology, Part B: Applications, Volume 314
Wang, D. Lu, P. Reaves, and M. Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences.
This volume will be a truly important tool in biomedically-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry.
Since , each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than volumes all of them still in print , the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Biochemists, geneticists, pharmacologists, oncologists, cell biologists, developmental biologists, molecular biologists, biomedically oriented researchers, neuroscientists, and physiologists.
It should be on the shelves of all libraries in the world as a whole collection. We are always looking for ways to improve customer experience on Elsevier.
kirichenko-music.com.ua/includes/contactos-gays/gozo-contactos-gay.php We would like to ask you for a moment of your time to fill in a short questionnaire, at the end of your visit. If you decide to participate, a new browser tab will open so you can complete the survey after you have completed your visit to this website. Thanks in advance for your time.
Skip to content. Search for books, journals or webpages All Pages Books Journals. View on ScienceDirect. Serial Volume Editors: M.
Ian Phillips. Imprint: Academic Press. Published Date: 22nd October Page Count: View all volumes in this series: Methods in Enzymology. Flexible - Read on multiple operating systems and devices. Easily read eBooks on smart phones, computers, or any eBook readers, including Kindle. When you read an eBook on VitalSource Bookshelf, enjoy such features as: Access online or offline, on mobile or desktop devices Bookmarks, highlights and notes sync across all your devices Smart study tools such as note sharing and subscription, review mode, and Microsoft OneNote integration Search and navigate content across your entire Bookshelf library Interactive notebook and read-aloud functionality Look up additional information online by highlighting a word or phrase.
- After 24 Years Of Struggle, Has Stan Crooke Finally Birthed An Age Of Antisense??
- Antisense Research and Application : Stanley T. Crooke : !
- Bibliographic Information.
- The Martha Stewart Living Cookbook: The Original Classics.
- The Indian Army, 1939-47: Experience and Development!
- Double Life (The Adventures of Josiah Jones Book 1).
- The Other in Contemporary Migrant Cinema.
Institutional Subscription. Free Shipping Free global shipping No minimum order. Antisense Receptor Targets: J. Antisense Neuroscience Targets: W.